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mda231 breast cancer cell line  (ATCC)


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    ATCC mda231 breast cancer cell line
    Migration assays were performed using the Boyden chamber method. After transfection of each siRNA into the (A) A549 and (B) <t>MDA231</t> cells, the cells were incubated for 48 h before the migration assay. The cells that had migrated to the outer side of the membranes within 24 h were fixed and stained. A representative image of a triplicate analysis of each siRNA is shown. Scale bar, 20 µm. siRNA, small interfering RNA; Ctrl, control siRNA; SERF2, small EDRK-rich factor 2; PRKDC, DNA-dependent protein kinase catalytic subunit; S100A6, S100 calcium-binding protein A6; SH3GLB1, SH3-domain GRB2-like endophilin B1; CAPNS1, calpain, small subunit 1; PSMB4, proteasome subunit β type-4; RPL11, ribosomal protein L11; RPS7, ribosomal protein S7.
    Mda231 Breast Cancer Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 24904 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mda231 breast cancer cell line/product/ATCC
    Average 99 stars, based on 24904 article reviews
    mda231 breast cancer cell line - by Bioz Stars, 2026-05
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    1) Product Images from "Exploration of genes related to the development of cancer of unknown primary"

    Article Title: Exploration of genes related to the development of cancer of unknown primary

    Journal: Oncology Reports

    doi: 10.3892/or.2025.8905

    Migration assays were performed using the Boyden chamber method. After transfection of each siRNA into the (A) A549 and (B) MDA231 cells, the cells were incubated for 48 h before the migration assay. The cells that had migrated to the outer side of the membranes within 24 h were fixed and stained. A representative image of a triplicate analysis of each siRNA is shown. Scale bar, 20 µm. siRNA, small interfering RNA; Ctrl, control siRNA; SERF2, small EDRK-rich factor 2; PRKDC, DNA-dependent protein kinase catalytic subunit; S100A6, S100 calcium-binding protein A6; SH3GLB1, SH3-domain GRB2-like endophilin B1; CAPNS1, calpain, small subunit 1; PSMB4, proteasome subunit β type-4; RPL11, ribosomal protein L11; RPS7, ribosomal protein S7.
    Figure Legend Snippet: Migration assays were performed using the Boyden chamber method. After transfection of each siRNA into the (A) A549 and (B) MDA231 cells, the cells were incubated for 48 h before the migration assay. The cells that had migrated to the outer side of the membranes within 24 h were fixed and stained. A representative image of a triplicate analysis of each siRNA is shown. Scale bar, 20 µm. siRNA, small interfering RNA; Ctrl, control siRNA; SERF2, small EDRK-rich factor 2; PRKDC, DNA-dependent protein kinase catalytic subunit; S100A6, S100 calcium-binding protein A6; SH3GLB1, SH3-domain GRB2-like endophilin B1; CAPNS1, calpain, small subunit 1; PSMB4, proteasome subunit β type-4; RPL11, ribosomal protein L11; RPS7, ribosomal protein S7.

    Techniques Used: Migration, Transfection, Incubation, Staining, Small Interfering RNA, Control, Binding Assay

    Western blotting showing the reduction in protein expression following siRNA-induced knockdown of PRKDC and PSMB4. (Top) Protein expression was reduced after transfection of the cells with siRNA for PRKDC (left) and PSMB4 (right) in both the A549 and MDA231 cells. β-actin was used as the loading control, whereas si-NC was used as the negative control. (Bottom) Relative reductions in PRKDC or PSMB4 protein expression were determined using western blot analysis conducted in triplicate. Data are presented as the mean and standard deviation. *P<0.05; **P<0.01. siRNA/si, small interfering RNA; si-NC, siRNA universal negative control; PRKDC, DNA-dependent protein kinase catalytic subunit; PSMB4, proteasome subunit β type-4.
    Figure Legend Snippet: Western blotting showing the reduction in protein expression following siRNA-induced knockdown of PRKDC and PSMB4. (Top) Protein expression was reduced after transfection of the cells with siRNA for PRKDC (left) and PSMB4 (right) in both the A549 and MDA231 cells. β-actin was used as the loading control, whereas si-NC was used as the negative control. (Bottom) Relative reductions in PRKDC or PSMB4 protein expression were determined using western blot analysis conducted in triplicate. Data are presented as the mean and standard deviation. *P<0.05; **P<0.01. siRNA/si, small interfering RNA; si-NC, siRNA universal negative control; PRKDC, DNA-dependent protein kinase catalytic subunit; PSMB4, proteasome subunit β type-4.

    Techniques Used: Western Blot, Expressing, Knockdown, Transfection, Control, Negative Control, Standard Deviation, Small Interfering RNA



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    Migration assays were performed using the Boyden chamber method. After transfection of each siRNA into the (A) A549 and (B) MDA231 cells, the cells were incubated for 48 h before the migration assay. The cells that had migrated to the outer side of the membranes within 24 h were fixed and stained. A representative image of a triplicate analysis of each siRNA is shown. Scale bar, 20 µm. siRNA, small interfering RNA; Ctrl, control siRNA; SERF2, small EDRK-rich factor 2; PRKDC, DNA-dependent protein kinase catalytic subunit; S100A6, S100 calcium-binding protein A6; SH3GLB1, SH3-domain GRB2-like endophilin B1; CAPNS1, calpain, small subunit 1; PSMB4, proteasome subunit β type-4; RPL11, ribosomal protein L11; RPS7, ribosomal protein S7.

    Journal: Oncology Reports

    Article Title: Exploration of genes related to the development of cancer of unknown primary

    doi: 10.3892/or.2025.8905

    Figure Lengend Snippet: Migration assays were performed using the Boyden chamber method. After transfection of each siRNA into the (A) A549 and (B) MDA231 cells, the cells were incubated for 48 h before the migration assay. The cells that had migrated to the outer side of the membranes within 24 h were fixed and stained. A representative image of a triplicate analysis of each siRNA is shown. Scale bar, 20 µm. siRNA, small interfering RNA; Ctrl, control siRNA; SERF2, small EDRK-rich factor 2; PRKDC, DNA-dependent protein kinase catalytic subunit; S100A6, S100 calcium-binding protein A6; SH3GLB1, SH3-domain GRB2-like endophilin B1; CAPNS1, calpain, small subunit 1; PSMB4, proteasome subunit β type-4; RPL11, ribosomal protein L11; RPS7, ribosomal protein S7.

    Article Snippet: The A549 human lung cancer cell line (cat. no. CCL-185) and the MDA231 breast cancer cell line (cat. no. HTB-26) were procured from American Type Culture Collection, and cultured in DMEM (Thermo Fisher Scientific, Inc.) with 10% FBS (MilliporeSigma) in accordance with the instructions provided by the suppliers.

    Techniques: Migration, Transfection, Incubation, Staining, Small Interfering RNA, Control, Binding Assay

    Western blotting showing the reduction in protein expression following siRNA-induced knockdown of PRKDC and PSMB4. (Top) Protein expression was reduced after transfection of the cells with siRNA for PRKDC (left) and PSMB4 (right) in both the A549 and MDA231 cells. β-actin was used as the loading control, whereas si-NC was used as the negative control. (Bottom) Relative reductions in PRKDC or PSMB4 protein expression were determined using western blot analysis conducted in triplicate. Data are presented as the mean and standard deviation. *P<0.05; **P<0.01. siRNA/si, small interfering RNA; si-NC, siRNA universal negative control; PRKDC, DNA-dependent protein kinase catalytic subunit; PSMB4, proteasome subunit β type-4.

    Journal: Oncology Reports

    Article Title: Exploration of genes related to the development of cancer of unknown primary

    doi: 10.3892/or.2025.8905

    Figure Lengend Snippet: Western blotting showing the reduction in protein expression following siRNA-induced knockdown of PRKDC and PSMB4. (Top) Protein expression was reduced after transfection of the cells with siRNA for PRKDC (left) and PSMB4 (right) in both the A549 and MDA231 cells. β-actin was used as the loading control, whereas si-NC was used as the negative control. (Bottom) Relative reductions in PRKDC or PSMB4 protein expression were determined using western blot analysis conducted in triplicate. Data are presented as the mean and standard deviation. *P<0.05; **P<0.01. siRNA/si, small interfering RNA; si-NC, siRNA universal negative control; PRKDC, DNA-dependent protein kinase catalytic subunit; PSMB4, proteasome subunit β type-4.

    Article Snippet: The A549 human lung cancer cell line (cat. no. CCL-185) and the MDA231 breast cancer cell line (cat. no. HTB-26) were procured from American Type Culture Collection, and cultured in DMEM (Thermo Fisher Scientific, Inc.) with 10% FBS (MilliporeSigma) in accordance with the instructions provided by the suppliers.

    Techniques: Western Blot, Expressing, Knockdown, Transfection, Control, Negative Control, Standard Deviation, Small Interfering RNA